Schematic Representation of Shot, its isoforms, and deletion constructs. (A) Schematic diagram of Shot A is given. The Shot A, B, and C isoforms vary at their NH2 terminus while having identical central and COOH-terminal domains. The Shot C isoform differs having only one CH domain. ShotΔRod, ShotΔRodΔC, ShotΔC, and ShotΔGAS2 are derived from Shot A. All constructs are COOH EGFP-tagged unless otherwise noted.