Figure 5.

Reduced amounts of TOM complex in strains lacking Mdm10. (A) Mitochondria from the indicated strains were subjected to BNGE, blotted to PVDF membrane, and decorated with Tom40 antibodies. Different amounts of control mitochondria were loaded to allow estimation of the degree of deficiency in the mutants. (B) Import of Tom40 precursor into differing amounts of wild-type mitochondria and standard amounts (50 μg) of mutant mitochondria. Further processing was as described in the legend to Figure 2B. (C) As in B, except that the radiolabeled precursor of porin was imported.