Figure 8.

Analysis of mitochondrial outer membrane phospholipids in the Δmdm10 mutant. OMV were isolated from either wild-type or Δmdm10 mutant cells. Phospholipids were extracted and analyzed by TLC and staining with molybdenum blue. Lane number is indicated at the bottom of the figure. Marker lipids were analyzed in parallel (lanes 1–3). Lanes 4–7, protein concentration in OMVs was measured, and lipids were extracted from samples equivalent to either 270 μg (1X prot) or 540 μg (2X prot) of both wild-type and mutant OMV. Lanes 8–11, Phosphate concentration in OMVs was measured and lipids were extracted from samples equivalent to either 167 μg (1X phos) or 334 μg (2X phos) of both wild-type and mutant OMV. PC, phosphatidylcholine; PE, phosphatidylethanolamine; CL, cardiolipin.