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. 2010 May 15;21(10):1737–1752. doi: 10.1091/mbc.E09-08-0706

Figure 4.

Figure 4.

The receptor can polarize in the absence of Myo2 function. (A) Representative fluorescent images of Ste2-GFP localization, F-actin polarity, and GFP-Sec4 polarization in myo2 temperature-sensitive and MYO2 isogenic control strains. G1-synchronized cells were treated with 30 nM pheromone at the permissive temperature (0 time for the 23°C cultures) or 5 min after the shift to restrictive temperature (0 time for the 37°C cultures). Images were acquired every 15 min. Arrows indicate Ste2-GFP crescents. (B) Quantification of Sec4-GFP and actin patch polarity in myo2-16 and MYO2 cells at the permissive and restrictive temperatures. The bar graphs represent the percentages of cells exhibiting clear Sec4-GFP (light gray) and actin patch (dark gray) polarity at each time point (32 ≥ n ≥ 8). At 37°C, none of the cells showed actin patch polarity and only one cell showed Sec4-GFP polarity. (C) Quantification of Ste2-GFP polarization in myo2-16 and MYO2 cells at the permissive and restrictive temperatures. The bar graphs represent the mean polarity index ± SD at each time point (n = 15). *p < 0.001 and **p < 0.0001 for the comparisons of treated cells to 0 min.