Figure 2.

TPB2 gene silence by hairpin RNA expression. (A) Schematic map of hairpin RNA constructs. Open arrows represent TPB2 gene with double lines inside indicating the region targeted by the hairpin RNA. (B) TPB2 gene silencing by hairpin RNA expression. Northern blot of total RNA samples extracted from conjugating cells (4, 6, 8, 10, 12, 14, and 16 h after postmixing) transformed with the hairpin RNA construct with or without inducing with 0.05 μg/ml CdCl₂ in 10 mM Tris buffer. The approximate size of TPB2 mRNA is indicated to the left of the panels, and the right arrow indicates a new band likely from the degraded RNA. The robust TPB2 hairpin RNA expression was also detected. The RPL21 mRNA was used as an internal control to quantify relative folds of the full-length TPB2 mRNA level.