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The loss of either Spa2p (A) or Mss4p (B) function has no effect on mitochondrial inheritance in ptc1Δ mutant and wild-type cells. RFP fluorescence labeled mitochondria (Mito-RFP), Hmg1p-GFP–labeled ER and DIC images in representative small-budded cells are shown. Open arrowheads point to small buds. Bars, 2 μm. (C and D) The percentage of small buds containing a mitochondrial segregation structure is presented. Error bars, SEM of three experiments.
