Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Prostate Cancer Prostatic Dis. 2010 Jan 12;13(2):151–161. doi: 10.1038/pcan.2009.59

Figure 4. Fluorescent micrographs of AS and AI prostate cancer cells following freeze exposure with and without integrin inhibition.

Figure 4

Total levels of necrotic and apoptotic cell death were evaluated in LNCaP LP (A) and LNCaP HP (B) cells treated with 40μg/ml anti-α6β4 integrin antibody. Samples were frozen at −15°C and triple-probe fluorescent micrographs were taken after 3h, 6h, and 24h using Hoechst (blue) to assess viable cells, propidium iodide (red) to assess necrotic cells, and YO-PRO-1 (green) to assess apoptotic cells. A) Compared to freeze alone, AS cell line LNCaP LP with function-blocked integrins showed only slight increases in necrotic and apoptotic cells. B) Function blocked LNCaP HP exhibited significant increases (p < 0.05) in necrotic and apoptotic cell death at every tested time point, indicating that AI cell lines more greatly depended on integrin signaling pathways for increased freezing resistance.