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. Author manuscript; available in PMC: 2010 Dec 1.

Published in final edited form as: Prostate Cancer Prostatic Dis. 2010 Jan 12;13(2):151–161. doi: 10.1038/pcan.2009.59

Caspase-8 (A), Caspase-9 (B), and Caspase-3 (C) activities were determined for LNCaP LP, LNCaP HP, PC-3 AR, and PC-3 cell lines treated with an α6β4 integrin function blocking assay. Cells were frozen and cell lysates were collected at regular intervals up to 24-hours post-freeze. Caspase activity was based on the ability to convert non-fluorescent substrate to its cleaved, fluorescent form. Compared to freeze alone, integrin function blocked AS cell lines LNCaP LP and PC-3 AR exhibited slightly increased levels of caspase-activity that peaked after 3-hours post-freeze. Interestingly, integrin function blocked AI cell lines LNCaP HP and PC-3 exhibited significantly (p < 0.05) greater levels of caspase activity (compared to freeze alone) that peaked after 6-hours post-freeze. These data indicated that integrin function blocking induced differentially greater cell death responses in AI cell lines. Data presented represents 3 experimental repeats (N=3).

Caspase-8 (A), Caspase-9 (B), and Caspase-3 (C) activities were determined for LNCaP LP, LNCaP HP, PC-3 AR, and PC-3 cell lines treated with an α6β4 integrin function blocking assay. Cells were frozen and cell lysates were collected at regular intervals up to 24-hours post-freeze. Caspase activity was based on the ability to convert non-fluorescent substrate to its cleaved, fluorescent form. Compared to freeze alone, integrin function blocked AS cell lines LNCaP LP and PC-3 AR exhibited slightly increased levels of caspase-activity that peaked after 3-hours post-freeze. Interestingly, integrin function blocked AI cell lines LNCaP HP and PC-3 exhibited significantly (p < 0.05) greater levels of caspase activity (compared to freeze alone) that peaked after 6-hours post-freeze. These data indicated that integrin function blocking induced differentially greater cell death responses in AI cell lines. Data presented represents 3 experimental repeats (N=3).