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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Gene Ther. 2010 Feb 18;17(5):634–642. doi: 10.1038/gt.2010.1

Figure 1. Cross-reactivity of positively selected CD4+ splenocytes from HAdΔE1E3-, PAdΔE1E3-, BAdΔE1E3-, HAd-WT-, PAd-WT-, BAd-WT-, or mock-inoculated mice.

Figure 1

Splenocytes were positively selected with anti-CD4 monoclonal antibody-coated magnetic beads and were stimulated with HAdΔE1E3-, PAdΔE1E3-, BAdΔE1E3-, or mock-infected cell lysate for 20 h. The number of cells expressing IFNγ (A) or IL-4 (B) was measured by enzyme-linked immunospot (ELISPOT) assay. (C) The percentage of CD4+ cells expressing IFNγ was measured by flow cytometry assay. Values are reported as the average ± standard deviation for five animals per group. *P < 0.005 versus values at mock stimulation within each treatment group. †P < 0.005 for homologous stimulation versus heterologous stimulation within each treatment group.