Fig. 4.

Reverse transcription-PCR analysis for GAD65 and GAD67 mRNA expression in the naïve, allodynic and non-allodynic groups 2 weeks following neuropathic surgery. Behavioral differences among the groups are illustrated in Fig. 3A. (A) GAD65 and GAD 67 mRNA expression in the S1 spinal segments excised from the naïve, allodynic and non-allodynic animals. ATF3 mRNA expression in the S1 DRG was adopted as a marker of nerve injury. (B) Ipsilateral expression of GAD65 and GAD67 mRNA. Data are normalized to GAPDH and expressed as a ratio to naïve. Expressions of GAD65- and GAD67 mRNA in the ipsilateral hemi-cords of the allodynic and non-allodynic groups are not different from those of the naïve group.