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. Author manuscript; available in PMC: 2010 May 14.
Published in final edited form as: Alcohol Clin Exp Res. 2008 Mar 11;32(5):806–813. doi: 10.1111/j.1530-0277.2008.00638.x

Fig. 7.

Fig. 7

Effect of ethanol and estradiol on pituitary Gi23 protein contents in ovariectomized (Ovx) and estradiol-treated ovariectomized animals (Ovx-E2). Rats were fed an ethanol diet (AF) or an isocaloric control diet (PF) for 2 or 4 weeks. The pituitary level of Gi3 was measured by Western blot. The tissue level of actin was used as loading control. Upper panel shows the representative blots. Lower panel shows the ratio of band intensities of Gi3 protein and actin. Each bar represents mean ± SEM of 4 to 6 different animals. *p < 0.05, compared with PF-fed Ovx group; **p < 0.05, compared with AF-fed E2 group.