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. Author manuscript; available in PMC: 2010 May 14.
Published in final edited form as: J Endocrinol. 2007 Feb;192(2):395–403. doi: 10.1677/joe.1.06925

Figure 2.

Figure 2

Colocalization of TSP-1 immunoreactivity with CD34 immunoreactivity in the anterior pituitary: effect of estradiol. (A–C) Representative photographs showing colocalization of TSP-1 and CD34 immunoreactivity in the pituitary of an ovariectomized rat with an empty implant for 4 weeks. TSP-1 immunofluorescence staining is shown in red (A) and CD34 is in green (B) and doubled labeled cells are shown in yellow (C). (D and E) Representative photograph showing immunofluorescence staining of TSP-1 and CD34 in anterior pituitaries of ovariectomized rat with an estradiol implant for 2 (D) or 4 weeks (E). Some of the double-labeled cells are shown by arrows. Immunofluorescence staining was absent when the section was stained in the absence of primary antibodies (F). Scale bar is 20 μM.