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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Stroke. 2009 Sep 17;40(11):3618–3626. doi: 10.1161/STROKEAHA.109.561951

Figure 6.

Figure 6

Shh is produced by mature neurons and inhibition decreases ischemia-induced proliferation. Shh/NeuN or GFAP double immunohistochemistry 7 days after ischemia. Almost all Shh+ cells (red) except for a few (noted by arrows) colocalize the mature neuronal marker NeuN (green) and no cells localize with GFAP (green). Cyclopamine blocks ischemia-induced NPC proliferation in the subgranular zone. A, Vehicle group demonstrates ischemia-induced proliferation compared to sham. Cyclopamine (60 pmol/day) blocked the ischemia-induced proliferation (mean ± SD, n=4). B, (cyclopamine) and C (vehicle), representative photomicrographs of BrdU immunohistochemistry after ischemia of the subgranular zone demarcated by line (Bar=100µm).