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. 2010 Apr 28;91(6):1634–1641. doi: 10.3945/ajcn.2009.28836

FIGURE 2.

FIGURE 2

A and B: Effect of zinc on nuclear transcription factor κB (NF-κB) activation in THP-1 cells after oxidized LDL (oxLDL) or phorbol myristate acetate (PMA) stimulation. Zinc-deficient (Zn−) THP-1 cells and zinc-sufficient (Zn+) THP-1 cells were used for the measurement of NF-κB activation by electrophoretic mobility shift assay (EMSA; A) and luciferase reporter gene assay (B). C and D: Effect of zinc on NF-κB activation in human aortic endothelial cells (HAECs) after oxLDL stimulation. Zn− HAECs and Zn+ HAECs were used for the measurement of NF-κB activation by EMSA (C) and luciferase reporter gene assay (D). *P < 0.05 for Zn− compared with Zn+ (n = 3). AFU, arbitrary fluorescent unit/β-galactosidase U/100 μg protein; P.C., positive control; C.C., competition control.