Figure 2.

Relationship between liposome volume and efficiency of selection. Two types of GFP genes, GFPuv5 and GFPuv2 (GFPuv5:GFPuv2=0.85:0.15), were encapsulated into a liposome population having a diverse size range together with a cell-free transcription-translation system. GFPuv5 shows eightfold higher fluorescence signal than GFPuv2. Following translation, liposomes showing high GFP fluorescence were collected with a fluorescent-activated cell sorter, and investigated the copy number of total GFP genes and the ratio of the GFPuv5 gene to the GFPuv2 gene in the collected liposome population.