Table 3.

Human Growth Hormone was measured in blood and urine at the same time from healthy donors.

Healthy Donor hGH +	Blood (ng/ml)	Direct Measurement in Urine by the Immunoassay¶ (ng/ml)	Particle Eluate (ng/ml)	Calculated actual urine concentration of hGH following nanoparticles harvesting (Particle Eluate / Concentration Factor) (pg/ml)
1	2.830	<0.050	0.107	0.973
2	0.095	<0.050	0.056	0.509
3	10.136	<0.050	0.078	0.100
4	1.880	<0.050	0.058	0.527
4 repeat	2.04	<0.050	0.063	0.572

⁺Measurements were done using the Immulite hGH serum Immunoassay (Siemens) with a lower detection limit of 50 pg/ml and a linear range of 50 pg-40 ng per ml.

^¶Prior to nanoparticle sequestration of hGH from the urine, the concentration of hGH in all urine samples was below the detection limit (<0.050 ng/mL) of the immulite assay.

The actual concentration in urine was calculated as (particle eluate concentration) / CF, where CF is the Concentration Factor = (Starting volume of urine) / (Volume of eluate) = 33ml/0.3 ml = 110.