Phylogenetic tree of PUUV sequences from the investigated rodents from South-East Germany in comparison to other Eurasian hantaviruses. The phylogenetic tree is based on a 712 nt fragment of the viral S segment (nt 364–1076). Figures on each branch represent the percentage of bootstrap support for maximum parsimony (100×bootstrap). Bootstrap values >50 are indicated. Puumala viruses are shaded. Abbreviations: N-Scand., North Scandinavia; S-Scand., South Scandinavia (Norway, Sweden), NW-Germany, North-West Germany; SE-Germany, South-East Germany. Abbreviations for strains: Hantaan, Hantaan virus strain 76–118, M14626; Saaremaa, Apodemus agrarius-associated Saaremaa/160V virus, AJ009773; Dobrava-Af, Dobrava virus strain associated to Apodemus flavicollis, L41916; SEO, Seoul virus strain SR-11, M34881; Tula-G1, Tula virus strain Germany 1, AF164093; Tula-G2, Tula virus strain Germany 2, AF164094; Tula-Mor, Tula virus strain Moravia/5302Ma/94, Z49915; Tula-Slo, Tula virus strain Slovakia, AJ223600; Khabarovsk, Khabarovsk virus strain MF-43, U35255; Topografov, Topografov virus strain Ls136V, AJ011646; Tobetsu, PUUV strain Tobetsu 60Cr/93, AB010731; Kamiiso, PUUV strain Kamiiso 8Cr/95, AB010730; Fyn, PUUV strain Fyn, AJ238791; Fyn47, PUUV strain Fyn 47, AJ278092; Omsk, PUUV strain Omsk/CG144/199-2000, AF367064; Puu-1324, PUUV strain 1324CG/79, Z46942; Gomselga, PUUV strain Karelia/Gomselga, AJ238790; Virrat, PUUV strain Virrat/25Cg/95, Z69985; Evo, PUUV strain Evo/12Cg/93, Z30702; Mellansel, PUUV strain Mellansel/Cg47/94, AJ223374; Vindeln, PUUV strain Vindeln/L20Cg/83, Z48586; Hundberget, PUUV strain Hundberget/Cg36/95, AJ223371; Vranica, PUUV strain Vranica/Hällnäs, U14137; Tavelsjo, PUUV strain Tavelsjo Cg81/94, AJ223380; Bashkiria, PUUV strain Bashkortostan/2001/CG17B, AF442613; P360, PUUV strain P360, L11347; CG18-20, PUUV strain CG18-20/Ufa83/Häallnaäs B1, M32750; Samara, PUUV strain Samara/FS-808 AF411446; Kazan, PUUV strain Kazan, Z84204; Udm-4, PUUV strain Udmurtia 444Cg/88, Z30706; Eidsvoll, PUUV strain Eidsvoll 1124v, AJ223368; Solleftea, PUUV strain Puu/Solleftea/Cg3/95, AJ223376; Balkan-1, PUUV strain Balkan 1, AJ314600; Balkan-2, PUUV strain Balkan 2, AJ314601; Slovakia, PUUV virus strain Opina916-Slovakia, AF294652; Berkel, PUUV strain Berkel, L36943; Paris, PUUV strain Paris 90-13 CG13891, U22423; Erft-G, PUUV strain Erft Germany, AJ238779; Thuin, PUUV strain Thuin AJ277030; Couvin, PUUV strain Couvin, AJ277034; Momignies, PUUV strain Momignies, AJ277032.