Figure 1.

Experiment outline. A cell lysate of 1205Lu cell line was processed in such a manner that each common step was comparable. Details of the methods are described in the Materials and Methods. The 2-D method consisted of SDS-PAGE and LC-MS/MS. The same samples were reanalyzed another three times as a repetitive analysis strategy. The 3-D method consisted of MicroSol IEF, SDS-PAGE, and LC-MS/MS. Subsequently, the final minimum consensus protein lists from the three data sets were compared to evaluate the ability of enhancing sensitivity of repetitive analysis and additional fractionation strategies.