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. 2009 Dec 16;9(2):1032–1040. doi: 10.1021/pr900927y

Figure 2.

Figure 2

Separation of the melanoma 1205Lu cell extract by MicroSol IEF and pooling to produce four fractions. Equal portions of each fraction and separation membrane disk extract were analyzed by SDS-PAGE to evaluate separation and relative amounts of total protein in each fraction. The solution recovered from individual chambers was pooled with the adjacent membrane disk extract on the low pH side of the pool, as shown at the bottom of the gel, to produce four fractions. The extract from the pH 12 membrane disk had negligible protein and was not used further.