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Cd promotes translocation of ERα to the nucleus. MCF-7 cells were plated in six-well plates containing untreated glass cover-slips under hormone-deprived conditions. Twenty hours later, cells were treated with 10−6m CdCl2 or 10−8m 17β-E2. Cells were harvested by fixation in 10% formalin 0, 0.5, 2.0, and 4.0 h after treatment. ERα (FITC) and nuclear staining (DAPI) were analyzed by immunofluorescence.
