Figure 6.—
The HMR-I silencer and tRNAThr gene help maintain silenced chromatin at HMR when deacetylase activity is reduced. (A) Sir3p association with HMR in the presence of either wild-type Sir2p alone (LRY1007) or Sir2p and catalytically inactive Sir2-N345Ap (LRY0804). Values in A–D represent the averages of at least three independent experiments analyzed as in Figure 1B. (B) Sir3p association with HMR-ΔtRNAThr in the presence of Sir2p alone (LRY2302) or Sir2p and Sir2-N345Ap (LRY2303). (C) Sir3p association with HMR-ΔI in the presence of Sir2p alone (LRY2315) or Sir2p and Sir2-N345Ap (LRY2316). (D) Sir3p association with HMR-ΔI ΔtRNAThr in the presence of Sir2p alone (LRY2309) or Sir2p and Sir2-N345Ap (LRY2352). (E) The relative enrichments of Sir2p and Sir3p with telomere VI-R and the modified HMR locus in the presence of Sir2-N345Ap (LRY2352). Data were analyzed as in Figure 1, D and E.