TABLE 1.
Strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| LRY1007 (W303) | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | R. Rothstein |
| LRY0800 | W303 MATα sir2Δ∷TRP1 LEU2∷sir2-N345A | |
| LRY0804 | W303 MATα LEU2∷sir2-N345A | |
| LRY1021 | MATahis4 | P. Schatz |
| LRY1068 | W303 MATα sir2Δ∷TRP1 | |
| LRY1815 | W303 MATα hmr-ΔI LEU2∷sir2-N345A | |
| LRY2148 | W303 MATα TELVIR∷STUFFER | |
| LRY2150 | W303 MATα TELVIR∷HMR-E | |
| LRY2302 | W303 MATα HMR-ΔtRNAThr | |
| LRY2303 | W303 MATα HMR-ΔtRNAThrLEU2∷sir2-N345A | |
| LRY2309 | W303 MATα hmr-ΔI ΔtRNAThr | |
| LRY2352 | W303 MATα hmr-ΔI ΔtRNAThrLEU2∷sir2-N345A | |
| LRY2315 | W303 MATα hmr-ΔI | |
| LRY2316 | W303 MATα hmr-ΔI LEU2∷sir2-N345A | |
| LRY2379 | W303 MATα [pJR831; PGAL1-HO; URA3]a | |
| LRY2384 | W303 MATα hmr-ΔI [pJR831; PGAL1-HO; URA3]a | |
| LRY2467 | W303 MATa [pJR831; PGAL1-HO; URA3]a | |
| LRY2482 | W303 MATα sir4Δ∷HIS3 [pJR831; PGAL1-HO; URA3]a | |
| YAB65 | W303 MATα hmr-ΔE (546–30; 516-bp deletion) | A. Brand |
| YAB71 | W303 MATα hmr-ΔE (358–223; 135-bp deletion) | A. Brand |
a
Brackets denote transformation with the indicated plasmid. See materials and methods for plasmid details.