Figure 2.—

Dosage suppression of Δbud3 growth and septation defects by rho4. (A–C) Colony morphology of Δbud3 strain AHS3 transformed with multiple copies of cdc42 (A), rac1 (B), or rho4 (C) and grown on selective MN media. Only rho4 functions as a dosage suppressor and restores conidiation. Hyphal morphology of Δbud3 strain AHS3 transformed with vector control (D) or rho4 (E). Hyphae were grown on YGV medium for 14 hr and stained with Calcofluor and Hoechst 33258 to visualize septa and nuclei, respectively. The presence of rho4 restored septum formation (arrows) to the ΔAnbud3 mutant. Bar, 10 μm.