Figure 8.—

Flies doubly mutant for ds2p and dscap exhibit phenotypes similar to ds2p single mutants. Virgin ds2p²/ds2p² females were crossed to ds2p¹/CyO males. Virgin dscap⁴/dscap⁴ females were crossed to dscap⁴/CyO males. For the dscap ds2p double mutants, virgin ds2p² dscap⁴/ds2p² dscap⁴ females were crossed to ds2p¹ dscap⁴/CyO, actin-GFP males. Heterozgous dsrebp¹⁸⁹ males were crossed to females of the same genotype. (A) Rescue of ds2p, dscap double mutants by dietary supplementation. For each experiment, 10 vials were set up at 1 mg embryos/vial on standard medium and an additional 10 vials of each cross were set up on medium supplemented with 0.075% myristate and 0.15% oleate (C14:0 + C18:1). Shown is the mean of three independent experiments. Error bars represent the SEM. A mean of 1415 adults (range, 872–1973) were scored for each genotype and condition. (B) Lipid content of wild-type and mutant flies reared on standard medium. Lipid mass was determined as described in materials and methods. The mean of five independent determinations is shown. Error bars represent the SEM.