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Fitness and copy number of ileS in lineages of C12 evolved in LB (A) or LB containing 100 μg/ml of mupirocin (B). Wild-type fitness in LB was set to 1.0 and the relative fitness of the strains was calculated as the ratio of tgen(wt)/tgen(mutant). Bars represent relative ileS DNA levels; circles are relative growth rates in LB (solid) or LB containing 100 μg/ml of mupirocin (open).
