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. 2010 May 14;5(5):e10656. doi: 10.1371/journal.pone.0010656

Figure 1. Distamycin A attenuates endotoxin-induced lung and liver inflammation.

Figure 1

A. Lungs were harvested from C57BL/6 male mice 24 hours following treatment with Vehicle (Veh), LPS/Vehicle (LPS+Veh) or LPS/Dist A (LPS+Dist A) i.p. (n = 9 mice per group). Lungs were fixed, processed, sectioned, and stained for Gr-1 (a marker of neutrophils, positive staining indicated by brown cells). Representative sections for each condition are shown (200× magnification). Using NIH Image Software, 5 fields (at 200× magnification) per lung section from mice from each treatment group were quantified, with brown pixels counted as positive staining. Results were expressed as % positively stained area per 200× field. (*p<0.05 compared with Veh; **p<0.05 compared with LPS+Veh). B. Liver tissue was harvested from C57BL/6 mice 4 h after treatment with Vehicle (Veh), LPS/Vehicle (LPS+Veh) or LPS/Dist A (LPS+DistA) i.p., (n = at least 4 mice per group) then processed, stained for Gr-1 (neutrophil marker), and analyzed as described above for Fig. 1A. (*p<0.05 compared with Veh; **p<0.05 compared with LPS+Veh).