Figure 2. Distamycin A attenuates inflammatory cytokine-induced neutrophil-endothelial interactions.

A. Wild type mice were subjected to intravital microscopy analysis at 3–4 hours following treatment with TNF-α/DistA or TNF-α/Vehicle (Veh) (n = 3 mice per treatment group with multiple vessels and vascular segments analyzed for each animal). Representative still photos of vascular segments are shown for the 4-hour time point. White round spots lining the vascular wall represent adherent leukocytes. B. Formal video analysis of the phases of neutrophil-endothelial interaction was performed, and values for all vascular segments within a particular treatment group were averaged for each time point. A significant reduction in rolling fraction and sticking efficiency was detected in the TNF-α/Dist A mice at 3–4 hours (p = 0.0001 and 0.0004, respectively), compared with the TNF-α/Vehicle mice.