Figure 5.

Rescue of noncholinergic neurotransmitter expression by blockade of muscle activity. A, Diagram illustrating hKir mRNA injection and neuron–muscle coculture with silenced muscle and wild type neurons. B, Diagram showing method of pharmacological blockade of muscle activity by α-bungarotoxin. C, Complete rescue of GABA expression and partial rescue of glycine and glutamate expression are achieved by either BgTx incubation or Kir expression. Lower asterisks indicate a significant difference between the bar beneath and its counterpart on the blank side. Upper asterisks indicate a significant difference between the bars (n > 5 cultures per condition per transmitter, >100 neurons per culture; *p < 0.05, **p < 0.01, ***p < 0.001). Error bars indicate SEM. The Kruskal–Wallis test and Conover post hoc test were used to determine statistical significance.