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Blockade of trk receptors does not rescue expression of noncholinergic transmitters. K252a (10 nm) was applied from 5 to 24 h. No significant difference in expression of GABA, glycine, or glutamate was observed between the blank and muscle sides of the dish (n > 5 cultures per condition per transmitter, >100 neurons per culture). Higher concentrations (up to 10 μm) were also ineffective. Error bars indicate SEM. The Kruskal–Wallis test was used to determine statistical significance.
