Table 1.

Effect of HIF-1α or HEF1 silencing on genes up-regulated by hypoxia. LS174T cells transfected with non-targeting HIF-1α, or HEF1 siRNA were exposed to normoxia or hypoxia for 12 hrs and then analyzed by RT-qPCR for changes in hypoxia-inducible gene expression. Results shown are expressed as fold increase of hypoxia versus normoxia. The values represent the mean of three different experiments done in triplicate ± SD. §, not significant.

Sun-Hee Kim

Target gene	Non-targeting siRNA	HIF-1α siRNA	HEF1 siRNA
Integrin α-2	12.02 ± 2.62	3.64 ±0.52	2.54 ± 0.43
PLAC8	4.77±0.25	3.080 ±0.08	1.85 ± 0.22
ELF3	14.80 ± 1.08	1.52 ±0.03	6.06 ± 0.15
STC1	14.94 ± 0.53	2.86 ±0.17	6.81 ± 0.22
ADM	34.42 ±4.88	9.99 ±2.37	20.17 ± 1.84
MKNK2	9.60 ± 0.50	1.94 ±0.08	5.01 ± 0.49
PFKP	4.20 ± 0.55	1.30 ±0.15	2.27 ± 0.29
PAI	13.03 ± 0.47	3.12 ±0.57	7.18 ± 0.50
ADFP	12.59 ± 1.94	2.69 ±0.52	7.12 ± 1.60
ALOXE3	4.260 ± 0.32	1.28 ±0.16	2.49 ± 0.10
VEGF	15.260 ± 1.57	6.39 ±1.98	8.57 ± 0.75
BMP2	8.60 ± 0.50	2.73 ±0.33	5.15 ± 0.32
KIT	3.93 ± 0.09	1.25 ±0.08	2.42 ± 0.18
CA9	18.85 ± 2.72	3.54 ±0.26	11.72 ± 1.88
MMP9	2.42 ± 0.24	1.56±0.24	1.51± 0.23
FABP1	8.86 ±0.32	3.07 ±0.30	6.11 ± 0.49
PCK1	63.21 ± 5.06	29.98 ±2.25	40.61 ± 2.73
NRN1	8.01 ± 0.08	2.76 ±0.49	5.17 ± 0.54
FOS	27.840 ± 1.26	12.81 ±0.58	16.89 ± 0.68
ALOX5	2.73 ± 0.07	1.19 ±0.11	1.760 ±0.08
DDIT4	12.60 ± 1.06	5.35 ±0.36	8.79 ± 0.05
BHLHE40	24.20 ± 1.09	10.03 ±0.56	17.360 ±0.07
AOAH	5.190 ± 0.19	2.48 ±0.15	3.79 ± 0.13
LRP1	9.28 ± 0.40	1.03 ± 0.09	6.82 ± 0.34
GDF15	9.00 ± 0.46	5.55 ±0.33	7.70 ± 0.26
BNIP2	8.60 ±4.51	2.14 ±0.42	4.70 ±0.58
TXNIP	6.59±1.06	1.81 ±0.29	5.06 ±0.54§
ERRFI1	22.24 ± 1.29	9.01 ±0.22	20.80 ± 0.34§
ANKRD37	57.83 ± 6.00	19.44 ±2.61	54.20 ± 4.49§
PLOD2	12.02 ± 3.09	3.21 ±0.37	11.80 ± 0.89§
PGK1	5.78 ±1.10	1.63 ±0.15	4.92 ± 0.29§
PPP1R3B	4.580 ± 0.53	2.7 ±0.33	4.52 ± 0.03§
SLC2A	21.632 ± 2.45	5.80 ±0.63	18.57 ± 0.17§