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NLRX1 is undetectable at the mitochondrial outer membrane. Purified mitochondria (M) were fractioned by ultracentrifugation into mitoplasts (MP), which are enriched in inner-membrane proteins but still contain outer-membrane proteins, and pure outer membrane (OM). The presence of the endogenous NLRX1, as well as known mitochondrial markers, was analyzed by western blotting.
