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. 2009 Mar 3;122(7):912–918. doi: 10.1242/jcs.039024

Fig. 7.

Fig. 7.

Coassembly of flotillin-1 Y160F and flotillin-2 Y163F produces microdomains that are not competent for internalisation. (A) Use of cell fusion to study assembly of flotillins into microdomains and consequent endocytosis. A flotillin-1-GFP-expressing cell has fused with a cell expressing flotillin-2-YFP. Assembly of the two proteins into microdomains at the area of plasma-membrane fusion has occurred. Merged image is shown on the right. Image acquired 30 minutes after initiation of fusion reaction. Scale bar: 20 μm. (B) Endocytic structures containing flotillins and CD59 are generated in the region of cell fusion when cells expressing wild-type flotillins fuse, but not when the tyrosine mutants are expressed instead. Basal confocal sections (z=0 μm) demonstrate that microdomain assembly occurs normally in both cases; higher (z=2 μm) sections demonstrate presence or absence of CD59 and flotillin-positive endosomes (arrowed) in the region of cell fusion. Scale bars: 5 μm.