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The effects of soluble JH8194 on the proliferation of MC3T3-E1 cells were investigated by MTS assay. The cells were cultured in the presence or absence of soluble JH8194 for four days. Soluble JH8194 was added to each well just once until the following assay. The MTS assay was done to examine the effects of soluble JH8194 on MC3T3-E1 proliferation. Independent experiments were repeated three times. Data represent the means +/– S.D of quadruplicate experiments.
