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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: J Cell Physiol. 2009 Apr;219(1):45–56. doi: 10.1002/jcp.21649

Figure 6.

Figure 6

Glomerular mesangial cell adhesion depends on endogenous Pyk2 activity. A. Representative example of three independent experiments to analyze endogenous Pyk2 phosphorylation either during GMC spreading or in suspension. Endogenous Pyk2 phosphorylation, as assessed by Western blotting with anti-phospho-Tyr402 antibody, was increased during GMC spreading on plate. Introduction of CRNK construct into GMC reduced Pyk2 phosphorylation. B. Illustration of decreased adhesion of AdGFP and AdCRNK-infected human glomerular mesangial cells (GMC) 1h after plating on regular cell culture treated plastic plates before and after washing unbound cells. Pictures were taken either under fluorescent (left panels) or bright field conditions. C. Summary of three independent experiments with GMC adhesion to Fibronectin or BSA (negative control) 30 min and 1h after plating. P<0.05 was considered significant and marked as * on the graph.