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. Author manuscript; available in PMC: 2011 Mar 5.
Published in final edited form as: Circ Res. 2010 Jan 7;106(4):720–729. doi: 10.1161/CIRCRESAHA.109.204396

Figure 3. Upregulation of proteins involved in endosomal cholesterol trafficking in FTY720 treated cells.

Figure 3

Representative Western blots and quantification of NPC1, NPC2, LAMP1 and ABCA1 levels from macrophages. Cells were treated for 24 h ± 1 μmol/L FTY720. Then, foam cell formation was induced by addition of 50 μg/mL acLDL for 24 h. At this stage, not loaded (-) and acLDL-loaded (acLDL) cells were harvested. A parallel set of acLDL loaded macrophages were allowed to undergo cholesterol efflux to 10 μg/mL apoA-I for 24 h (acLDL→apoA-I) before harvesting for analysis. Bars show the relative quantities of the proteins from at least 3 independent donors.