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. Author manuscript; available in PMC: 2011 Mar 5.
Published in final edited form as: Circ Res. 2010 Jan 7;106(4):720–729. doi: 10.1161/CIRCRESAHA.109.204396

Figure 6. BODIPY-FTY720 trafficking in NPC1-deficient cells.

Figure 6

Wild-type and NPC1-deficient (M12) CHO cells were labeled with 1 mg/mL rhodamine-dextran for 24 h followed by incubation with 1 μmol/L BODIPY-cholesterol or 1 μmol/L BODIPY-FTY720 for 24 h and chasing for 0 h or 24 h. Living cells were imaged and BODIPY intensity in dextran-positive organelles was quantified directly after loading (0 h) and following the 24 h chase. Quantification from eight independent measurements and representative fluorescence micrographs are shown. Scale bar, 10 μm.