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. Author manuscript; available in PMC: 2011 Mar 5.
Published in final edited form as: Circ Res. 2010 Jan 7;106(4):720–729. doi: 10.1161/CIRCRESAHA.109.204396

Figure 8. Mechanism for FTY720 induced upregulation of ABCA1.

Figure 8

A. Macrophages were treated for 24 h ± 1 μmol/L FTY720 together with 10 μmol/L T0901317 or 5 μmol/L UVI3003 as indicated. Representative Western blot and quantification of ABCA1 levels from three donors in duplicate. B. Control siRNA or NPC1 siRNA treated macrophages were treated ± 1 μmol/L FTY720 for 24 h. The cellular levels of NPC1 and ABCA1 were analyzed by Western blotting and ABCA1 was quantified from three individual donors in duplicate. C. Macrophages were treated for 24 h ± 1 μmol/L FTY720. Surface proteins were biotinylated, pulled down with streptavidin agarose, and surface ABCA1 analyzed by Western blotting. Ont-tenth of cell lysate was removed before pull-down of the biotinylated proteins to reflect the total cellular amount of ABCA1. The level of ABCA1 on the surface was 15-20% of total ABCA1 in both control and FTY720 treated cells. Macrophages from a total of five donors were analyzed. D. Proposed model of FTY720 action in macrophage atheroprotection. 27-HC, 27-hydroxycholesterol. The thick arrows indicate the intracellular routing of cholesterol.