Visualization by confocal laser scanning microscopy of focal adhesions and cytoskeleton of hFOB 1.19 cells after 1 day (a, d, g), 7 days (b, e, h), and 14 days (c, f, i) of seeding on autoclaved (a–c), thermally oxidized at 500°C (d–f), and at 800°C (g–i) Ti–6Al–4V surfaces. Cells exhibited microspikes (dashed arrows), stress fibers (arrows), focal contacts at the cell periphery or at the end of the stress fibers (arrowheads). Scale bar: 50 μm