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Mass calibration drift for precursors (a) and products (b) over 60 replicate LC-MS/MS runs performed after external mass calibration. A Gaussian fit was applied to the mass error distribution for each run before (red) and after linear (green) and proportional (blue) internal mass recalibration; error bars represent ±1 S.D. For both MS1 and MS2 scans, proportional internal mass recalibration virtually eliminated drift and reduced the width of the distributions.
