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. 2010 Feb 11;9(5):838–850. doi: 10.1074/mcp.M900489-MCP200

Fig. 6.

Fig. 6.

Separation, sequencing, and quantification of isobaric modified peptides. The extracted ion chromatograms of the doubly charged precursor ion (m/z 739.4333) from the “light” dimethylated peptide H3.3 KSAPSTGGVKKPHR (aa 27–40) (upper) and the corresponding “heavy” peptide m/z 751.4544 (2+) (lower). Separation of these isobaric modified peptides allowed quantification of the two different dimethylated peptide species. The dimethylated peptide “KSAPSTGGVKme2KPHR” eluted prior to the isobaric dimethylated peptide “Kme2SAPSTGGVKPHR.” The filled circle is wild type (light isotope version), and the empty circle is Suz12−/− (heavy isotope version). WT, wild type.