Table II. Prohead protease-processed segments of 201φ2-1 virion proteins.
| gpa | Slice | Residues |
Molecular massb | SCc | SC/molecular massd | |
|---|---|---|---|---|---|---|
| Start | Stop | |||||
| kDa | ||||||
| 148 | 25 | 114e | 414 (C)f | 34.9 | 134 | 3.8 |
| 151N | 45 | 1 | 112 | 11.9 | 91 | 7.6 |
| 151C | 28 | 167e | 400 (C) | 26.4 | 262 | 9.9 |
| 154 | 17 | 80 | 426 (C) | 40.3 | 46 | 1.1 |
| 155N | 27 | 14e | 275 | 27.8 | 67 | 2.4 |
| 155Na | 27 | 14e | 139 | 13.1 | 67 | 5.1 |
| 155C | 25 | 328e | 608 (C) | 30.7 | 378 | 12.3 |
| 156N | 46 | 86 | 157 | 8.0 | 23 | 2.9 |
| 156C | 28 | 158 | 387 (C) | 25.5 | 135 | 5.3 |
| 157 | 24 | 208 | 489 (C) | 31.4 | 225 | 7.2 |
| 158 | 24 | 61e | 378 (C) | 34.9 | 48 | 1.4 |
| 159 | 25 | 631 | 922 (C) | 31.3 | 369 | 11.8 |
| 193 | 28 | 140e | 381 (C) | 27.2 | 472 | 17.4 |
| 200 | 10 | 162 | 746 (C) | 65.1 | 2045 | 31.4 |
| 214N | 33 | 1 | 208 | 22.9 | 23 | 1.0 |
| 214C | 9 | 217 | 917 (C) | 78.6 | 64 | 0.8 |
| 238 | 27 | 65e | 316 (C) | 28.6 | 165 | 5.8 |
| 246N | 32 | 17e | 128e | 11.7 | 95 | 8.1 |
| 246M | 43 | 129 | 203e | 7.8 | 35 | 4.5 |
| 246C | 17 | 258e | 613 (C) | 38.7 | 761 | 19.7 |
| 247 | 21 | 51e | 397 (C) | 38.8 | 191 | 4.9 |
| 268 | 29 | 1 | 225 | 24.7 | 99 | 4.0 |
| 271 | 10 | 61e | 629 (C) | 63.6 | 396 | 6.2 |
| 274/3N | 25 | 1 | 275e | 32.5 | 35 | 1.1 |
| 274/3C | 4 | 276 | 1500 (C) | 137.2 | 327 | 2.4 |
| 280N | 46 | 16e | 72 | 6.1 | >7 | >1.1 |
| 280C | 40 | 73e | 199 (C) | 13.5 | 100 | 7.4 |
| 455N | 40, 41 | 21e | 151e | 15.0 | 17 | 1.1 |
| 455M | 26 | 180e | 453 | 30.7 | 44 | 1.4 |
| 455C | 28 | 521 | 768 (C) | 28.5 | 140 | 5.3 |
a The gene product name is appended with N (for N-terminal), M (for middle), or C (for C-terminal) when more than one segment of a gene product was detected in the virion. gp155N and gp155Na are alternative interpretations of the N-terminal gp155 segment (see text).
b The molecular mass was calculated from the sequence.
c SCs were summed over all slices. The peak of gp280N may be off the bottom of the gel.
d SC/molecular mass values were calculated as a rough indicator of abundance of the polypeptides per virion according to Ref. 13. Based on comparisons of values for the major capsid protein, sheath protein, and tape measure protein, a value of SC/molecular mass over 10 probably represents more than 100 molecules per virion.
e Confirmed by a semitryptic spectrum. Semitryptic spectrum assignments were above 95% confidence except as noted in Table I.
f A coordinate is appended with (C) for an end defined by the stop codon.