FIGURE 1.

Loss of CYLD causes CARMA1-independent NF-κB activation and IL-2 gene expression in thymocytes. A, cell lysates were prepared from thymocytes of WT, CARMA1^−/−, CYLD^−/−, or CARMA1^−/−CYLD^−/−dKO mice. IB was performed to detect CARMA1, CYLD, and the loading control tubulin. B, nuclear extracts were prepared from the indicated mouse strains and subjected to EMSA using ³²P-radiolabeled probes for NF-κB or the control nuclear protein, NF-Y. C, total RNA was isolated from the thymocytes of the indicated mouse strains and subjected to IL-2 real-time PCR. Data are presented as mean ± S.D. of fold of induction compared with the WT sample.