FIGURE 6.

Effect of overexpression of the Listericin gene on growth of infected bacteria in vivo. At 24 h after injection of L. monocytogenes (Lm (WT)), L. monocytogenes (Δhly), E. coli, Ecc 15, S. aureus, and S. saprophyticus into Collagen (Cg)-Gal4-driven GFP as a control, Listericin gene-overexpressed flies were sterilized by ethanol on the surface, homogenized, and diluted serially in the appropriate medium. Bacterial growth in each fraction was quantified by determining CFU by plate assay. Data represent the mean of at least three independent experiments with more than 30 flies of each genotype examined. Bars indicate S.D. *, p < 0.05.