FIGURE 2.

Differential induction in HYAL mRNA between two orbital fibroblast strains. cDNA from orbital fibroblast strains OF1 and OF2 treated with 5 μm PGD₂ or vehicle (Untreated) for 4 h was assessed by qRT-PCR. There was a significant increase in mRNA expression for all three HYAL isoforms (HYAL1, HYAL2, and HYAL3) when OF1 was treated with 5 μm PGD₂ (***, p < 0.0001 compared with untreated). There was no significant increase in HYAL1 to -3 in PGD₂-treated OF2 cells (ns, compared with untreated). The induction of HYAL1, HYAL2, and HYAL3 mRNA in PGD₂-treated OF1 was significantly higher than PGD₂-exposed OF2 HYAL expression ($$$, p < 0.0001 for each respective HYAL). Results are expressed as the mean ± S.D. (error bars) (n = 3).