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SDS-PAGE (top) and native-PAGE (bottom) Western blots of WT and purified CBS mutants. The purified CBS enzymes (100 ng) were separated in a 10% SDS-PAGE gel under denaturing conditions (top) and in a 4–15% PAGE gel under native conditions (bottom). Following electrophoresis, the gels were transferred to polyvinylidene difluoride membranes and probed with a monoclonal anti-CBS antibody (Abnova).
