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Thermostability of purified WT and CBS mutants. The individual enzymes were gradually heated from 10 to 90 °C as described under “Experimental Procedures.” A, Tonset designates the temperature at which aggregates start to form, and Tm represents melting temperatures. B, an example of the thermostability analysis of WT CBS. Error bars denote the S.E. values and are derived from a minimum of three independent measurements.
