FIGURE 7.

Protein levels of three molecular chaperones, DnaK, GroEL, and DnaJ, in response to the absence or presence of chemical chaperones. The crude extracts (50 μg) of mutant CBS enzymes expressed in the presence or in the absence of the chemical chaperones were separated in 9% Tris-HCl SDS-PAGE gel, transferred to polyvinylidene difluoride membrane, and probed with monoclonal anti-DnaK antibody, monoclonal anti-GroEL antibody, or polyclonal anti-DnaJ antibody. Values under the respective Western blot bands represent the -fold of increase of the molecular chaperone protein levels in response to the presence of a given chemical chaperone with S.E. calculated from data for each CBS mutant.