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Hap1 is involved in different protein complexes. Hap1 isoforms (Hap1A and Hap1B) are distributed in the continuous sucrose density gradient fractions isolated from post-nuclear supernatant of adult wild-type mouse brain. Both Hap1 isoforms are seen in the low density fractions, but only Hap1A is present in the high density fractions. Other organelle marker proteins were also examined, as indicated. Input, post-nuclear supernatant loaded at half the volume of the fractions.
