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Chromophore geometry in the mutant variants. a, Ser-65–Tyr-66–Gly-67 in aceGFP (numbers/symbols shown in blue; oxidized Tyr-66 with sp2 hybridized Cα/Cβ) and aceGFP-G222E (numbers/symbols shown in red; native Tyr-66 with sp3 hybridized Cα/Cβ) (current study). b, Thr-65–His-66–Gly-67 in the reduced BFPsol (PDB ID: 2FWQ (32). c, Thr-65–Leu-66–Gly-67 in the EGFP variant (PDB ID: 1S6Z (31)).
