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. 2010 Mar 16;285(21):16012–16022. doi: 10.1074/jbc.M109.096412

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Reduced mass analysis of glycosylated, deglycosylated, and lectin-enriched recombinant human IgG2 antibody. The UV chromatogram of the reversed phase separations of reduced glycosylated, deglycosylated, and lectin-enriched antibody samples are shown in Panel 1, A–C, respectively. The glycosylated HC mass spectrum is shown in Panel 2, the light chain mass spectrum is shown in Panel 3 and the deglycosylated antibody HC spectrum is shown in Panel 4. The lectin-enriched antibody HC mass spectrum from reversed phase peak 1 (Panel 1, C) is shown in Panel 5 and the lectin-enriched antibody LC mass spectrum from reversed phase peak 2 (Panel 1, C) is shown in Panel 6. The theoretical masses for each species are listed in parenthesis below the observed masses.